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Image Search Results
Journal: Plastic and Reconstructive Surgery Global Open
Article Title: Cryopreservation of Stromal Vascular Fraction Cells Reduces Their Counts but Not Their Stem Cell Potency
doi: 10.1097/GOX.0000000000002321
Figure Lengend Snippet: Multicolor flow cytometry analysis demonstrated a reduced percentage of hematopoietic cells and an enrichment of stem cell progenitor cells in cryopreserved SVF samples. Fresh (A) and cryopreserved (−80°C) SVF cells (B) were stained with a 7-color flow cytometry panel, treated with a fixating red blood lysis buffer and analyzed by flow cytometry. Using the gradual gating strategy shown, only viable cells determined by low ViViD staining (an amine reactive viability dye) while discarding RBCs were analyzed for their surface marker expression. Discrimination between the CD45− and CD45+ cells enabled determination of the percent of the different hematopoietic cell types within the hematopoietic (CD45+) population and the percent of the stem cell progenitor cells (CD31−CD34+ or CD29+CD73+) within the nonhematopoietic (CD45−) population. ViviD indicates violet viability dye. FSC-A, forward scatter area; SSC-A, Side scatter area.
Article Snippet: The following mouse antihuman antibodies were used for flow cytometry staining:
Techniques: Flow Cytometry, Staining, Lysis, Marker, Expressing
Journal: Plastic and Reconstructive Surgery Global Open
Article Title: Cryopreservation of Stromal Vascular Fraction Cells Reduces Their Counts but Not Their Stem Cell Potency
doi: 10.1097/GOX.0000000000002321
Figure Lengend Snippet: Surface marker analysis demonstrates a reduction of hematopoietic cells and an enrichment of stem cell progenitor cell populations within SVF cells following cryopreservation. Fresh and cryopreserved SVF cells were analyzed using a 7-color flow cytometry panel, according to the gating strategy presented in Figure . The percent of SVF cells that were CD45+ (A), the percent of the different hematopoietic cell types within the hematopoietic (CD45+) population (B), and the percent of CD31−CD34+ (C) or CD29+CD73+ (D) within the nonhematopoietic (CD45−) population are shown. Data are presented as mean ± SD. * P < 0.05, ** P < 0.01. n.s., difference not statistically significant.
Article Snippet: The following mouse antihuman antibodies were used for flow cytometry staining:
Techniques: Marker, Flow Cytometry